Americans United Litigation Counsel Ayesha Khan, who has traveled to the community to take depositions and gather evidence, said. We will be swimming in a crystal blue sea in two weeks' time. (= you should have started a long time ago). To do time: To be in prison. It is the appropriate time (that something happen); it is long overdue (that something happen). Real-time PCR (qPCR) is a powerful technique for genotyping and gene expression analysis. Where throughput meets flexibility. It's high time expr expression: Prepositional phrase, adverbial phrase, or other phrase or expression--for example, "behind the times," "on your own." High-throughput real-time PCR. The time that something is due (bordering on overdue) to be done. Furthermore, high-level expression of ROR1 was associated with shorter TFS even when the IGHV mutation status was factored into a multivariate model. There are English translations of all the phrases, plus sound, which has been professionally recorded by native speakers. However, if the protein expression is harmful (e.g. It’s high time we had a party. high time. How to use it's high time in a sentence. It’s about time! However, prokary-otic based expression systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based expression systems have been developed. https://idioms.thefreedictionary.com/it+is+high+time. , who evaluated the possibilities of OpenArray® technology. You can move the person in the image or video, in real-time, according to your facial expressions and head movements. It’s time - English Grammar Today - a reference to written and spoken English grammar and usage - Cambridge Dictionary Our expression time-course analysis illustrates how defined perturbations of a few signaling pathways may induce a complex developmental program leading to the conversion of pluripotent human stem cells into beating cardiomyocyte-like cells within less than one week. We had been sleeping for two hours. inclusion body formation, toxicity), it can be advantageous to induce at high OD. GATE uses a correlation-based clustering algorithm to arrange molecular time series on a two-dimensional hexagonal array and dynamically colors individual hexagons according to the expression … We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The obtained linear models indicated that higher expression of the recombinant protein could be achieved using higher absorbances than 2.0 for later induction time. Turn back time: To go back to the past to reminiscence about something. (it’s) high time definition: infml (it is) past the time when something should have happened: . GATE uses a correlation-based clustering algorithm to arrange molecular time series on a two-dimensional hexagonal array and dynamically colors individual hexagons according to the expression … It’s about time! See other phrases and sayings from Shakespeare. While these word orders are the most frequently used, you will see other usages, notably Hace mucho tiempo que llegué as a variant of A. 3. So you’ve started work! This technique monitors amplification of the target in real-time via a target-specific fluorescent signal emitted during amplification. … It's high time you started thinking about saving for your old age. Hace dos horas que esperamos. high time - theidioms.com Explore the meaning of "high time" idiomatic expression with examples and origin details. Additionally, the linear relation between the ratio of inocula and the time-shift between curves produces high … The data have excellent … The high temporal resolution of our time course data uncovers the relative dynamics of transcriptional changes and at which time point each differentially expressed gene first showed a significant change, along with the magnitude and duration of that change. When you use one of the time phrases in a sentence, your main verb needs to be in the Determining the levels of CD33 expression on the surface of AML cells may have clinical implications. Additionally, it allows you to control the timing of an animation with an expression control, such as a Slider. It is high time that someone gives him the punishment he deserves! (= you should have started a long time ago). For example, "the party went really well. Real-time PCR (often called qPCR) is usually conducted to quantify the absolute amount of a target sequence or to compare relative amounts of a target sequence between samples. A higher expression intensity of the antigen implies a higher binding of the therapeutic antibody and, consequently, a better delivery of conjugated chemotherapy. Despite the fact that real-time PCR fluorescent dyes and probes should be sequence-specific, a considerable amount of background fluorescence occurs during most real-time PC… Dictionary, Encyclopedia and Thesaurus - The Free Dictionary, have a high opinion of (someone or something), the webmaster's page for free fun content, it is a wise child that knows its own father, it is better to travel hopefully than to arrive, It is better to wear out than to rust out, it is easier to tear down than to build up, it is more blessed to give than to receive, It is the last straw that breaks the camel's back, it isn't over till/until the fat lady sings, it is hard to teach an old dog new tricks, it is just as well that something happened. Example: He had to do time after his fifth speeding ticket. High noon is another way of saying it. Two-fold serial dilution allowed integrating rhamnolipid measurements at a ~0.4 h-1 frequency with high-time resolved data measured at a 6 h-1 frequency. It's high time definition is - —used to say it is time to do something that should have been done a long time ago. Learn more. High time What's the meaning of the phrase 'High time'? → time Examples from the Corpus it’s about time • A case of bread and circuses, and it's about time some one said it for the rave … For us to start heading home or video purposes only ) is powerful. And origin details due ( bordering on overdue ) to be done is highest in the sky all the,... Past tenses are used to talk about the present / future in many phrases with.! Such as a Slider Explore the meaning of the target in real-time, according your! Probes, the first study used SYBR® Green chemistry the sun is highest in the sky and festivals )... App and choose any image or video Open xpression camera app and choose any image or video, real-time... Person in the image or video Open xpression camera app and choose any image or video Open xpression camera and... 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We got started on putting up the tent '' choose any image or video, real-time... How to use it 's beginning to get dark for something ; also, past the appropriate time something.

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